Ethidium fluorescence assays. Part 1. Physicochemical studies
نویسندگان
چکیده
منابع مشابه
Physicochemical variations in DOM-synchronous fluorescence: Implications for mixing studies
Synchronous fluorescence (SF) spectra of dissolved organic matter (DOp/I) show promise for mixing studies and can resolve multiple river inputs to large water masses in a single fast measurement. This study examines the effects of physicochemical variables on DOM SF independent of mixing. Our results show that changes in the pH and metal ion concentration do not affect SF in the alkaline Cuyaho...
متن کاملMechanism of Ethidium Bromide Fluorescence Enhancement on
The mechanism of the enhancement of the fluorescence of ethidium bromide on binding to double helical RNA and DNA has been investigated. From an examination of the effect of different solvents on the fluorescence lifetime, quenching of fluorescence by proton acceptors, and the substantial lengthening of lifetime observed upon deuteration of the amino protons, regardless of the medium, we conclu...
متن کاملEvaluation of HLA-DR typing by ethidium bromide fluorescence.
Sixty individuals were typed for D-related human leukocyte antigens (HLA-DR) using two methods: the standard eosin exclusion method and a recently described one-color ethidium bromide technique. As a result, 88 of the 90 DR antigens detected by the standard technique were also detected by one-color fluorescence (sensitivity = 97.8 percent). However, of the 99 DR antigens identified by ethidium ...
متن کاملManaging evaporation for more robust microscale assays. Part 1. Volume loss in high throughput assays.
Evaporation is a well known issue when handling small liquid volumes. Here we present a review of microscale assays prone to evaporation and methods to make them more robust. Applications for these assays span from combinatorial chemistry to cell-biology where the stability of concentrations and osmolarity can be critical. A dimensionless evaporation number Ev is presented and used to character...
متن کاملManaging evaporation for more robust microscale assays . Part 1 : Volume loss in droplet based assays
Humidity in an Omnitray has been measured after closing the container to determine the time to achieve equilibration. An Omnitray has been modified to allow the insertion of a humidity probe tip and sealed using BlueTak. One hundred 10μL drops were placed in the Omnitray and the lid was either placed on the container or sealed using BlueTak. Recording of the humidity was effectuated by hand (fi...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Nucleic Acids Research
سال: 1979
ISSN: 0305-1048,1362-4962
DOI: 10.1093/nar/7.3.547